Design your own microbial assays in just a few clicks with GeneGlobe

Learn how to design custom digital PCR assays for bacteria, fungi, and viruses using GeneGlobe’s intuitive microbial assay designer. This tool allows you to create targeted assays based on taxonomy, genomic regions, and optional anchor sequences—no bioinformatics expertise required. Whether you're working with well-known pathogens or rare organisms, this video shows how easy it is to start your design and get precise results.

This tutorial walks you through the Custom dPCR Microbial Assay Designer—from logging in and selecting your target organism to submitting a design and receiving results. Discover how to create specific, contamination-checked assays with confidence.

What is the Custom dPCR Microbial Assay Designer?

The Custom dPCR Microbial Assay Designer on GeneGlobe helps researchers build highly specific assays tailored to their microbial targets. Supporting bacteria, fungi, and viruses, the tool draws on trusted NCBI database to design assays against 16S, ITS, and viral sequences. The user-friendly interface lets you input a design name, select domain type, and specify a taxonomy using scientific or common names. Optional anchor sequences give you control over target regions, and the designer automatically screens out closely related non-targets and common contaminants. Once submitted, the system applies over 40 design parameters to ensure accuracy, specificity, and performance—delivering results to your GeneGlobe dashboard within minutes to days, depending on complexity. Already available catalog assays are flagged so you can choose between new designs or proven lab-tested options. Take your assay development further by visiting our Custom digital PCR assays page for a fully tailored design experience.

Common questions about Custom dPCR Microbial Assays tool

Custom designed dPCR Microbial assays can be ordered for detection in the Green, Yellow, Orange, Red, and Crimson channels on the QIAcuity.

Depending on the submitted target the algorithm can take up to 48 hours to complete the design. The majority of designs should take less than 1 day.

Sequences ranging between 200 bp and 5000 bp can be used. Recommended are at least 500 bp when possible.

The design tool is not intended to create multiplex assay designs. Individual assay designs may be compatible in multiplex reactions, but depending on which microbes are included in the sample and the target region of the assay, incompatibilities must be taken into account. More details on multiplexing of assays can be found in the Microbial dPCR Handbook.

Yes. All QIAcuity dPCR Microbial DNA Detection Assays and Custom dPCR Microbial have the same recommended cycling conditions.

The restriction enzymes compatible with each specific custom assay design are listed in the Product Sheet.

No, a batch design function is not implemented. Only one design request at a time can be submitted.

No. The custom assay design tool can only be used to create hydrolysis probe-based dPCR Microbial assays.

No. While the Custom dPCR Microbial Assays are designed in silico using stringent criteria to ensure optimal performance, the resulting assay designs are not subsequently wet lab tested.

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Design your own microbial assays in just a few clicks with GeneGlobe

What is the Custom dPCR Microbial Assay Designer?

Common questions about Custom dPCR Microbial Assays tool

In which channels can I detect custom designed dPCR Microbial assays?

I submitted a custom assay design >24h ago and did not receive a design. What is the reason?

What length of the DNA anchor sequence can be used as input for the custom assay design tool?

Can I design multiplex assays with the custom assay design tool?

Are the cycling conditions for Custom dPCR Microbial assays the same as for the pre-designed dPCR Microbial DNA Detection assays?

With which restriction enzymes are the Custom dPCR Microbial assays compatible?

Can I use the custom assay design tool to start multiple designs at once?

Can the custom assay design tool be used to order custom Microbial assays for use with intercalating dye-based chemistry?

Are the Custom dPCR Microbial Assays wet lab tested?

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