The miRCURY LNA miRNA Probe PCR System enables sensitive, specific miRNA quantification and profiling via real-time PCR. Detection is based on a FAM- and dark quencher-labeled probe quencher-labeled hydrolysis probe. The system covers all steps involved in converting miRNA to cDNA and subsequent qPCR detection of miRNAs. The miRCURY LNA miRNA Probe PCR Assays have been validated using the QuantiNova Probe Master Mix; use of other reagents for qPCR will affect the results.

RNA Spike-In Kit for RT

The RNA Spike-In Kit enables quality control of the RNA isolation, cDNA synthesis and PCR amplification steps of miRCURY LNA miRNA Probe PCR experiments so you can identify any poor-quality samples early in the process and save time and reagents. The kit includes UniSp2, UniSp4 and UniSp5 RNA Spike-in Template Mix and the cel-miR-39-3p RNA Spike-in Template.

miRCURY LNA RT Kit

One universal reverse transcription kit for two detection systems. The miRCURY LNA RT Kit is used for both the miRCURY LNA miRNA PCR System that employs SYBR Green for detection, and the probe-based miRCURY LNA miRNA Probe PCR System. The kit includes two system-specific reaction buffers and all the necessary reagents to perform fast and convenient miRNA polyadenylation and reverse transcription in a single reaction step.

miRCURY LNA Probe PCR Kit

Optimized and dedicated for the miRCURY LNA miRNA Probe PCR System, this kit contains the high-performance QuantiNova Probe Master Mix and the miRCURY Probe Universal Reverse Primer. Combined with a miRCURY LNA miRNA Probe PCR Assay or a miRCURY LNA miRNA Probe PCR Panel, the chemistry enables miRNA detection via quantitative real-time PCR.

miRCURY LNA miRNA Probe PCR Assays

These primer and probe assays use miRNA-specific hydrolysis probes and miRNA-specific forward primers designed to detect mature miRNAs using the most up-to-date sequence information from miRBase 22. The miRCURY Probe Universal Reverse Primer is contained in the miRCURY Probe PCR Kit.

miRCURY LNA miRNA Probe PCR Panels

Ready-to-use, predesigned, single-use PCR panel plates to rapidly profile mature miRNAs focusing on the miRNome, specific pathways, certain sample types like serum/plasma, or in a user-designed custom panel.

GeneGlobe Data Analysis Center

This complimentary, web-based analysis tool guides you step-by-step via an easy wizard through the normalization and analysis of your data and generates publication-ready figures.

Spike-in assays

Product name	Control type	Used to control	Targeted template
UniSp2 miRCURY LNA miRNA Probe Assay	Spike-in control assay	Extraction efficiency	UniSp2 – synthetic RNA template, incl. in RNA Spike-In Kit, for RT – 339390
UniSp4 miRCURY LNA miRNA Probe Assay	Spike-in control assay	Extraction efficiency	UniSp4 – synthetic RNA template, incl. in RNA Spike-In Kit, for RT – 339390
UniSp5 miRCURY LNA miRNA Probe Assay	Spike-in control assay	Extraction efficiency	UniSp5 – synthetic RNA template, incl. in RNA Spike-In Kit, for RT – 339390
UniSp6 miRCURY LNA miRNA Probe Assay	RT control assay	Reverse transcription reaction	UniSp6 – synthetic RNA template, included in miRCURY LNA RT Kit – 339340
UniSp9 miRCURY LNA miRNA Probe Assay	IPC – interplate calibrator	PCR reaction	UniSp9 – synthetic DNA template. Spotted with assay on the plate. Only available on plates (panels)
cel-miR-39-3p miRCURY LNA miRNA Probe Assay	RT control assay	Reverse transcription reaction	UniSp6 – synthetic RNA template, incl. in RNA Spike-In Kit, for RT – 339390

Control assays

Product name	Target organism	Alternative names	NCBI reference
Control primer set, SNORD38B (hsa)	hsa	U38B; RNU38B	NR_001457
Control primer set, SNORD44 (hsa)	hsa	U44; RNU44	NR_002750
Control primer set, SNORD48 (hsa)	hsa	U48; RNU48	NR_002745
Control primer set, SNORD49A (hsa)	hsa	U49; U49A; RNU49	NR_002744
Control primer set, SNORA66 (hsa)	hsa	U66; RNU66	NR_002444
Control primer set, 5S rRNA (hsa)	hsa, mmu	V00589
Control primer set, U6 snRNA (hsa, mmu)	hsa, mmu, rno	U6	X59362
Control primer set, RNU5G snRNA (mmu, hsa)	mmu, hsa, rno	Rnu5a; U5a; Rnu5g	NR_002852
Control primer set, RNU1A1 (mmu, hsa)	mmu, hsa, rno	Rnu1a-1; Rnu1a1	NR_004411
Control primer set, SNORD65 (mmu)	mmu	U65; RNU65	NR_028541
Control primer set, SNORD68 (mmu)	mmu	U68; RNU68	NR_028128
Control primer set, SNORD110 (mmu)	mmu	U110; RNU110	NR_028547

hsa-miR-6732-5p miRCURY LNA miRNA Probe PCR Assay

Product Specification

Resources