PCR plate and master mix
Target List
AKT1
The best known AKT1 mutations are c.49G>A, p.E17K, a PH domain mutation that results in constitutive targeting of AKT1 to plasma membrane, and an activating mutation, c.145G>A, p.E49K.
AKT3
The mutation assay detects an activating AKT3 mutation c.511G>A, p.G171R.
PIK3CA
The most frequently detected PIK3CA gain-of-function mutations occur either in the kinase domain (T1025-G1049, such as H1047L and H1047R) that increase its activity or in the helical domain (P539-E545) which mimic activation by growth factors.
PIK3R1
The mutation assays detect two recurrent disinhibitory PIK3R1 mutations, c.1126G>A, p.G376R and c.1690A>G, p.N564D, the latter of which causes increased basal kinase activity and lipid binding.
PIK3R5
The mutation assay detects a recurrent oncogenic mutation in PIK3R5.
PTEN
The most commonly detected PTEN loss-of-function mutations either truncate the protein (such as K6fs*4, R130*, R130fs*4, R233*, P248fs*5, and V317fs*3) or cause phosphatase inactivation (such as those at R130 and R173).
STK11
The most commonly detected STK11 inactivation variants either are point mutations or truncate the protein.