Each QuantiTect Primer Assay is supplied as a lyophilized mix of forward and reverse primers for a specific gene. The primers are reconstituted in TE, pH 8.0 to give a 10x primer solution, which is then added to the master mix supplied with a QuantiFast, QuantiTect, Rotor-Gene, or FastLane Kit for SYBR Green detection. The master mix with primers is aliquoted into PCR tubes or wells, and the individual template samples are then added.

QuantiTect Primer Assays provide guaranteed results in gene expression analysis when used in combination with one of the following kits shown in the table.

Two-step and one-step qRT-PCR kits for use with QuantiTect Primer Assays
Two-step qRT-PCR	One-step qRT-PCR
QuantiFast SYBR Green PCR Kit	QuantiFast SYBR Green RT-PCR Kit
Rotor-Gene SYBR Green PCR Kit	Rotor-Gene SYBR Green RT-PCR Kit
QuantiTect SYBR Green PCR Kit	QuantiTect SYBR Green RT-PCR Kit

QuantiTect Primer Assays are designed to detect RNA only where possible. For assays where detection of genomic DNA cannot be avoided, cDNA can be prepared using the QuantiTect Reverse Transcription Kit or the FastLane Cell cDNA Kit. Both kits provide cDNA synthesis with integrated removal of genomic DNA contamination.

Naming

Each QuantiTect Primer Assay available for a particular gene is given a number that appears in the product name after the gene symbol. Read more in the GeneGlobe FAQs.

Hs_SPAG16_vb.1_SG QuantiTect Primer Assay

Product Specification

Resources